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Expression of microRNA‐184 in keratinocytes represses argonaute 2
Author(s) -
Roberts Julian C.,
Warren Richard B.,
Griffiths Christopher E.M.,
Ross Kehinde
Publication year - 2013
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24401
Subject(s) - hacat , argonaute , gene silencing , microrna , proinflammatory cytokine , microbiology and biotechnology , small interfering rna , cytokine , biology , keratinocyte , cell culture , transfection , immunology , inflammation , gene , genetics
ABSTRACT Interleukin‐22 (IL‐22) is a proinflammatory cytokine that has been associated with the pathogenesis of inflammatory skin disorders. However, the impact of IL‐22 on microRNA (miRNA) expression in epidermal keratinocytes is unknown. Here we show that IL‐22 induces miR‐184 in reconstituted human epidermis (RHE) and in the HaCaT keratinocyte cell line. Exposure to IL‐22 increased miR‐184 expression 8‐ and 15‐fold in RHE and HaCaT cells, respectively. Oncostatin M, an unrelated proinflammatory cytokine, also raised miR‐184 expression in RHE and HaCaT keratinocytes. Pharmacologic and genetic inhibition demonstrated that cytokine‐induced expression of miR‐184 was mediated by signal transducer and activation of transcription 3 (STAT3). Argonaute 2 (AGO2), a member of the RNA‐induced silencing complex (RISC), is a predicted miR‐184 target. Using protein, messenger RNA and reporter analyses, we found that miR‐184 regulates the expression of AGO2. We conclude that cytokine‐induced miR‐184 attenuates AGO2 expression in keratinocytes. J. Cell. Physiol. 228: 2314–2323, 2013. © 2013 Wiley Periodicals, Inc.