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Frequency‐dependent cell death by optical tweezers manipulation
Author(s) -
NG K.S.,
ZHOU Z.L.,
NGAN A.H.W.
Publication year - 2013
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24369
Subject(s) - optical tweezers , tweezers , programmed cell death , amplitude , cell , biophysics , atomic force microscopy , nanotechnology , optics , materials science , chemistry , optoelectronics , biology , apoptosis , physics , biochemistry
Optical tweezers were used to scan individual Chronic Myelogenous Leukemia cells to determine if the cell death depends on the scanning conditions. Although increasing the scanning frequency or amplitude means greater force applied to the cells, their effects on cell death are not a simple increasing trend, as observed in the optical microscopy. Indeed, cell death sharply increased at particular screening frequencies and amplitudes, whereas other frequencies or amplitudes were less detrimental. These results suggest that cell damage was more sensitive to certain scanning conditions, rather than simply high‐applied forces. J. Cell. Physiol. 228: 2037–2041, 2013. © 2013 Wiley Periodicals, Inc.