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Growth of v‐src‐transformed cells in serum‐free medium through the induction of growth factors
Author(s) -
DeAngelis Tiziana,
Quong Andrew,
Morrione Andrea,
Baserga Renato
Publication year - 2013
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24303
Subject(s) - proto oncogene tyrosine protein kinase src , osteopontin , oncogene , microbiology and biotechnology , biology , embryo , growth factor , 3t3 cells , cell , gene , chemistry , immunology , cell cycle , signal transduction , receptor , biochemistry , transfection
The v‐src oncogene is one of only two oncogenes capable of transforming mouse embryo fibroblasts (MEFs) lacking the IGF‐IR gene (R‐cells). R‐/v‐src cells grow robustly in the absence of serum, suggesting the hypothesis that they may produce one or more growth factors that would sustain their ability to proliferate in serum‐free condition. Using proteomic approaches on serum‐free conditioned media derived from v‐src‐transformed cells, we have identified two growth promoting factors: ostepontin and proliferin. Subsequent experiments have indicated that osteopontin plays a prevalent role in promoting growth of v‐src‐transformed cells in serum‐deprived condition. J. Cell. Physiol. 228: 1482–1486, 2013. © 2012 Wiley Periodicals, Inc.