Premium
Itch, an E3 ligase of Oct4, is required for embryonic stem cell self‐renewal and pluripotency induction
Author(s) -
Liao Bing,
Zhong Xiaoming,
Xu Huiming,
Xiao Feng,
Fang Zhuoqing,
Gu Junjie,
Chen Yue,
Zhao Yingming,
Jin Ying
Publication year - 2013
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24297
Subject(s) - ubiquitin ligase , reprogramming , embryonic stem cell , microbiology and biotechnology , biology , ubiquitin , stem cell , transcription factor , rex1 , gene silencing , regulator , cell , induced pluripotent stem cell , genetics , gene
Abstract Itch, a C2‐WW‐HECT domain ubiquitin E3 ligase, plays an important role in various biological processes. However, its role in embryonic stem cells (ESCs) remains unknown. Here, we report that Itch interacts with and targets pluripotency‐associated transcription factor Oct4 for ubiquitination. Moreover, Itch enhances Oct4 transcriptional activities and controls Oct4 protein stability dependent on its catalytic activity. Importantly, silencing Itch expression compromises ESC self‐renewal capacity and somatic cell reprogramming efficiency. Taken together, our study identifies Itch as a regulator of Oct4 stability and transcriptional activity, establishing a functional link between an E3 ligase and the regulation of pluripotency. J. Cell. Physiol. 228: 1443–1451, 2013. © 2012 Wiley Periodicals, Inc.