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FAK regulates Cdk2 in EGF‐stimulated primary cultures of hepatocytes
Author(s) -
Flinder Liv Ingrid,
Wierød Lene,
Rosseland Carola M.,
Huitfeldt Henrik S.,
Skarpen Ellen
Publication year - 2013
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24287
Subject(s) - microbiology and biotechnology , primary (astronomy) , cyclin dependent kinase 2 , primary culture , chemistry , epidermal growth factor , cancer research , kinase , biology , cell culture , protein kinase a , physics , genetics , astronomy
In this study, we report a novel role of FAK as a regulator of Cdk2 in anchorage‐dependent primary cultured hepatocytes. In response to EGF, we found that S‐phase entry was reduced upon FAK inhibition. This correlated with decreased protein expression and nuclear accumulation of the G1/S‐phase regulator Cdk2. Further, nuclear accumulation of the Cdk2 partner cyclinE, was reduced, but not its protein level. Also, protein levels of Cdk2 were inversely linked with increased expression of the Cdk2 inhibitor p27, known to be degraded in a Cdk2‐dependent manner. Also, cyclinD1 was regulated by FAK, but to a lesser extent than Cdk2. To assess the mechanism in which FAK mediates Cdk2‐regulation, FAK mutants were used: FAKY397F, mutated at its integrin‐regulated site, and two others mutated at docking sites for Grb2‐ERK‐activation (FAKY925F) and for p130Cas‐Rac1‐activation (FAKY861F). All three sites were central for EGF‐induced ERK‐activity and Cdk2 expression. In addition, FAK was important for HGF‐mediated proliferation, suggesting a general mechanism for anchorage‐dependent growth. Moreover, growth factor‐induced cell spreading, but not survival, required FAK. Hence, integrins and growth factors cooperate in anchorage‐dependent signaling events leading to proliferation and motility. In conclusion, our data suggest that FAK acts as a central coordinator of integrin and growth factor‐mediated S‐phase entry by its ability to regulate Cdk2. J. Cell. Physiol. 228: 1304–1313, 2013. © 2012 Wiley Periodicals, Inc.