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Angiopoietin 1 enhances the proliferation and differentiation of skeletal myoblasts
Author(s) -
Lee Eun Hui,
Woo Jin Seok,
Hwang JiHye,
Park JaeHyeong,
Cho ChungHyun
Publication year - 2013
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24251
Subject(s) - myogenesis , microbiology and biotechnology , myocyte , skeletal muscle , angiogenesis , chemistry , integrin , orai1 , biology , endocrinology , cell , cancer research , biochemistry , stim1 , endoplasmic reticulum
Angiopoietin 1 (Ang1) plays an important role in various endothelial functions, such as vascular integrity and angiogenesis; however, less is known about its function outside of the endothelium. In this study, we examined whether Ang1 has direct effects on skeletal muscle cells. We found that Ang1 exhibited myogenic potential, as it promoted the proliferation, migration, and differentiation of mouse primary skeletal myoblasts. The positive effect of Ang1 on myoblast proliferation could have been mediated by the α7 and β1 integrins. We also found that Ang1 potentiated cellular Ca 2+ movements in differentiated myotubes in response to stimuli, possibly through the increased expression of two Ca 2+ ‐related proteins, namely, Orai1 and calmodulin. Ang1 also increased Orai1 and calmodulin expression in mouse hearts in vivo. These results provide an insight into the molecular mechanisms by which Ang1 directly affects the myogenesis of striated muscle. J. Cell. Physiol. © 2012 Wiley Periodicals, Inc.