Premium
Transcription of the pain‐related TRPV1 gene requires Runx1 and C/EBPβ factors
Author(s) -
Ugarte Giorgia D.,
Diaz Emilio,
Biscaia Miguel,
Stehberg Jimmy,
Montecino Martin,
van Zundert Brigitte
Publication year - 2013
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24236
Subject(s) - trpv1 , transcription factor , runx1 , promoter , transcription (linguistics) , microbiology and biotechnology , gene , e box , gene expression , chemistry , transient receptor potential channel , biology , receptor , genetics , linguistics , philosophy
Transient Receptor Potential Vanilloid type 1 channel (TRPV1) is an important endogenous transducer of noxious heat and chemical stimuli and is required during development of inflammatory hypersensitivity. The transcription factor Runx1 is known to play a relevant role in sensory neuron differentiation as it controls the expression of several sensory nociceptive receptors, including TRPV1. Here, we show that Runx1 up‐regulates TRPV1 transcription activity by interacting directly with the proximal TRPV1 gene promoter sequence. Importantly, C/EBPβ a well‐established heterodimer partner of Runx1 also binds to the TRPV1 promoter and cooperates with Runx1 to further stimulate TRPV1 transcription. Our results support a mechanism where Runx1‐C/EBPβ‐containing transcription regulatory complexes are recruited to the TRPV1 gene promoter to modulate TRPV1 expression in dorsal root ganglia neurons. J. Cell. Physiol. 228: 860–870, 2013. © 2012 Wiley Periodicals, Inc.