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Vitamin C treatment promotes mesenchymal stem cell sheet formation and tissue regeneration by elevating telomerase activity
Author(s) -
Wei Fulan,
Qu Cunye,
Song Tieli,
Ding Gang,
Fan Zhipeng,
Liu Dayong,
Liu Yi,
Zhang Chunmei,
Shi Songtao,
Wang Songlin
Publication year - 2012
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24012
Subject(s) - periodontal ligament stem cells , mesenchymal stem cell , stem cell , sox2 , microbiology and biotechnology , regeneration (biology) , stem cell transplantation for articular cartilage repair , extracellular matrix , fibronectin , chemistry , homeobox protein nanog , biology , cellular differentiation , adult stem cell , embryonic stem cell , alkaline phosphatase , induced pluripotent stem cell , biochemistry , gene , enzyme
Cell sheet engineering has been developed as an alternative approach to improve mesenchymal stem cell‐mediated tissue regeneration. In this study, we found that vitamin C (Vc) was capable of inducing telomerase activity in periodontal ligament stem cells (PDLSCs), leading to the up‐regulated expression of extracellular matrix type I collagen, fibronectin, and integrin β1, stem cell markers Oct4, Sox2, and Nanog as well as osteogenic markers RUNX2, ALP, OCN. Under Vc treatment, PDLSCs can form cell sheet structures because of increased cell matrix production. Interestingly, PDLSC sheets demonstrated a significant improvement in tissue regeneration compared with untreated control dissociated PDLSCs and offered an effective treatment for periodontal defects in a swine model. In addition, bone marrow mesenchymal stem cell sheets and umbilical cord mesenchymal stem cell sheets were also well constructed using this method. The development of Vc‐mediated mesenchymal stem cell sheets may provide an easy and practical approach for cell‐based tissue regeneration. J. Cell. Physiol. 227: 3216–3224, 2012. © 2011 Wiley Periodicals, Inc.