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Dynamic compression of rabbit adipose‐derived stem cells transfected with insulin‐like growth factor 1 in chitosan/gelatin scaffolds induces chondrogenesis and matrix biosynthesis
Author(s) -
Li Jianjun,
Zhao Qun,
Wang Enbo,
Zhang Chuanhui,
Wang Guangbin,
Yuan Quan
Publication year - 2012
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22927
Subject(s) - chondrogenesis , chitosan , gelatin , adipose tissue , microbiology and biotechnology , transfection , matrix (chemical analysis) , chemistry , rabbit (cipher) , growth factor , stem cell , biology , biochemistry , gene , computer science , receptor , computer security , chromatography
Articular cartilage is routinely subjected to mechanical forces and growth factors. Adipose‐derived stem cells (ASCs) are multi‐potent adult stem cells and capable of chondrogenesis. In the present study, we investigated the comparative and interactive effects of dynamic compression and insulin‐like growth factor‐I (IGF‐I) on the chondrogenesis of rabbit ASCs in chitosan/gelatin scaffolds. Rabbit ASCs with or without a plasmid overexpressing of human IGF‐1 were cultured in chitosan/gelatin scaffolds for 2 days, then subjected to cyclic compression with 5% strain and 1 Hz for 4 h per day for seven consecutive days. Dynamic compression induced chondrogenesis of rabbit ASCs by activating calcium signaling pathways and up‐regulating the expression of Sox‐9. Dynamic compression plus IGF‐1 overexpression up‐regulated expression of chondrocyte‐specific extracellular matrix genes including type II collagen, Sox‐9, and aggrecan with no effect on type X collagen expression. Furthermore, dynamic compression and IGF‐1 expression promoted cellular proliferation and the deposition of proteoglycan and collagen. Intracellular calcium ion concentration and peak currents of Ca 2+ ion channels were consistent with chondrocytes. The tissue‐engineered cartilage from this process had excellent mechanical properties. When applied together, the effects achieved by the two stimuli (dynamic compression and IGF‐1) were greater than those achieved by either stimulus alone. Our results suggest that dynamic compression combined with IGF‐1 overexpression might benefit articular cartilage tissue engineering in cartilage regeneration. J. Cell. Physiol. 227: 2003–2012, 2012. © 2011 Wiley Periodicals, Inc.