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Mouse embryonic stem cells that survive γ‐rays exposure maintain pluripotent differentiation potential and genome stability
Author(s) -
Rebuzzini Paola,
Pignalosa Diana,
Mazzini Giuliano,
Di Liberto Riccardo,
Coppola Antonio,
Terranova Nadia,
Magni Paolo,
Redi Carlo Alberto,
Zuccotti Maurizio,
Garagna Silvia
Publication year - 2012
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22908
Subject(s) - embryonic stem cell , apoptosis , biology , induced pluripotent stem cell , stem cell , cell cycle , cell culture , microbiology and biotechnology , andrology , genetics , gene , medicine
This study aimed to investigate the cell cycle, apoptosis, cytogenetics and differentiation capacity of mouse embryonic stem cells (mESCs) that survived a single dose of 2 or 5 Gy γ‐rays during a period of up to 96 h of culture. After 2 Gy irradiation and 24 h culture, compared to control, a significant majority of cells was blocked at the G2/M phase and a massive apoptosis was recorded. Between 48 and 72 h post‐irradiation, the parameters used to describe the cell cycle and apoptosis returned similar to those of control samples. When mESCs were irradiated with 5 Gy, a small fraction of cells, even after 96 h of culture, still presented clear evidences of a G2/M block and apoptosis. The cytogenetic analysis performed at 96 h showed that the structural stability of the aberrations did not change significantly when comparing control and 2 or 5 Gy‐treated populations. However, the chromosomal damage observed in the progeny of the survived cells after 5 Gy exposure is significantly higher than that observed in control samples, although it is mostly of the stable and transmissible type. Ninety‐six hours after irradiation, the survived mESCs maintained their undifferentiated status and capability to differentiate into the three germ layers. Overall, these results indicate a commitment of mESCs to maintain pluripotency and genome stability. J. Cell. Physiol. 227: 1242–1249, 2012. © 2011 Wiley Periodicals, Inc.