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β Common receptor integrates the erythropoietin signaling in activation of endothelial nitric oxide synthase
Author(s) -
Su KuoHui,
Shyue SongKun,
Kou Yu Ru,
Ching LiChieh,
Chiang AnNa,
Yu YuanBin,
Chen ChienYu,
Pan ChingChian,
Lee TzongShyuan
Publication year - 2011
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22678
Subject(s) - erythropoietin receptor , enos , protein kinase b , phosphorylation , microbiology and biotechnology , erythropoietin , proto oncogene tyrosine protein kinase src , pi3k/akt/mtor pathway , chemistry , signal transduction , receptor , janus kinase 2 , erythropoiesis , nitric oxide synthase type iii , medicine , nitric oxide synthase , nitric oxide , biology , endocrinology , biochemistry , anemia
Erythropoietin (EPO), the key hormone for erythropoiesis, also increases nitric oxide (NO) bioavailability in endothelial cells (ECs), yet the definitive mechanisms are not fully understood. Increasing evidence has demonstrated that β common receptor (βCR) plays a crucial role in EPO‐mediated non‐hematopoietic effects. We investigated the role of βCR in EPO‐induced endothelial NO synthase (eNOS) activation in bovine aortic ECs (BAECs) and the molecular mechanisms involved. Results of confocal microscopy and immunoprecipitation analyses revealed that βCR was colocalized and interacted with EPO receptor (EPOR) in ECs. Inhibition of βCR or EPOR by neutralizing antibodies or small interfering RNA abolished the EPO‐induced NO production. Additionally, blockage of βCR abrogated the EPO‐induced increase in the phosphorylation of eNOS, Akt, Src, or Janus kinase 2 (JAK2). Immunoprecipitation analysis revealed that treatment with EPO increased the interaction between βCR and eNOS, which was suppressed by inhibition of Src, JAK2, or Akt signaling with specific pharmacological inhibitors. Furthermore, EPO‐induced EC proliferation, migration, and tube formation were blocked by pretreatment with βCR antibody and Src, JAK2, or PI3K/Akt inhibitors. Moreover, in vivo experiments showed that EPO increased the level of phosphorylated eNOS, Src, JAK2, and Akt, as well as βCR–eNOS association in aortas and promoted the angiogenesis in Matrigel plug, which was diminished by βCR or EPOR neutralizing antibodies. Our findings suggest that βCR may play an integrative role in the EPO signaling‐mediated activation of eNOS in ECs. J. Cell. Physiol. 226: 3330–3339, 2011. © 2011 Wiley Periodicals, Inc.