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Insulin regulates GLUT1‐mediated glucose transport in MG‐63 human osteosarcoma cells
Author(s) -
Cifuentes Manuel,
García Maria A.,
Arrabal Pilar M.,
Martínez Fernando,
Yañez María J.,
Jara Nery,
Weil Bernardo,
Domínguez Dolores,
Medina Rodolfo A.,
Nualart Francisco
Publication year - 2011
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22668
Subject(s) - glucose transporter , glut1 , osteosarcoma , medicine , glucose uptake , biology , endocrinology , insulin , cancer research , cancer cell , glut4 , cancer
Osteosarcoma is the most common type of malignant bone cancer, accounting for 35% of primary bone malignancies. Because cancer cells utilize glucose as their primary energy substrate, the expression and regulation of glucose transporters (GLUT) may be important in tumor development and progression. GLUT expression has not been studied previously in human osteosarcoma cell lines. Furthermore, although insulin and insulin‐like growth factor (IGF‐I) play an important role in cell proliferation and tumor progression, the role of these hormones on GLUT expression and glucose uptake, and their possible relation to osteosarcoma, have also not been studied. We determined the effect of insulin and IGF‐I on GLUT expression and glucose transport in three well‐characterized human osteosarcoma cell lines (MG‐63, SaOs‐2, and U2‐Os) using immunocytochemical, RT‐PCR and functional kinetic analyses. Furthermore we also studied GLUT isoform expression in osteosarcoma primary tumors and metastases by in situ hybridization and immunohistochemical analyses. RT‐PCR and immunostaining show that GLUT1 is the main isoform expressed in the cell lines and tissues studied, respectively. Immunocytochemical analysis shows that although insulin does not affect levels of GLUT1 expression it does induce a translocation of the transporter to the plasma membrane. This translocation is associated with increased transport of glucose into the cell. GLUT1 is the main glucose transporter expressed in osteosarcoma, furthermore, this transporter is regulated by insulin in human MG‐63 cells. One possible mechanism through which insulin is involved in cancer progression is by increasing the amount of glucose available to the cancer cell. J. Cell. Physiol. 226: 1425–1432, 2011. © 2011 Wiley‐Liss, Inc.