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Resistin induces monocyte–endothelial cell adhesion by increasing ICAM‐1 and VCAM‐1 expression in endothelial cells via p38MAPK‐dependent pathway
Author(s) -
Hsu WeiYen,
Chao YuWen,
Tsai YingLan,
Lien ChihChan,
Chang ChaoFu,
Deng MingChung,
Ho LowTone,
Kwok Ching Fai,
Juan ChiChang
Publication year - 2011
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22555
Subject(s) - resistin , icam 1 , monocyte , vcam 1 , cell adhesion molecule , cell adhesion , umbilical vein , intercellular adhesion molecule 1 , adhesion , microbiology and biotechnology , endothelial stem cell , chemistry , medicine , endocrinology , biology , biochemistry , adiponectin , in vitro , insulin , insulin resistance , organic chemistry
Resistin, firstly reported as an adipocyte‐specific hormone, is suggested to be an important link between obesity and diabetes. Recent studies have suggested an association between resistin and atherogenic processes. The adhesion of circulating monocytes to endothelial cells is a critical step in the early stages of atherosclerosis. The purpose of the present study was to investigate the effect of resistin on the adhesion of THP‐1 monocytes to human umbilical vein endothelial cells (HUVECs) and the underlying mechanism. Our results showed that resistin caused a significant increase in monocyte adhesion. In exploring the underlying mechanisms of resistin action, we found that resistin‐induced monocyte adhesion was blocked by inhibition of p38MAPK activation using SB203580 and SB202190. Furthermore, resistin increased the expression of intercellular adhesion molecule‐1 (ICAM‐1) and vascular cell adhesion molecule‐1 (VCAM‐1) by HUVECs and these effects were also p38MAPK‐dependent. Resistin‐induced monocyte adhesion was also blocked by monoclonal antibodies against ICAM‐1 and VCAM‐1. Taken together, these results show that resistin increases both the expression of ICAM‐1 and VCAM‐1 by endothelial cells and monocyte adhesion to HUVECs via p38MAPK‐dependent pathways. J. Cell. Physiol. 226: 2181–2188, 2011. © 2010 Wiley‐Liss, Inc.