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Leptin up‐regulates pro‐inflammatory cytokines in discrete cells within mouse colon
Author(s) -
Padidar Sara,
Farquharson Andrew J.,
Williams Lynda M.,
Kelaiditi Eirini,
Hoggard Nigel,
Arthur John R.,
Drew Janice E.
Publication year - 2011
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22546
Subject(s) - leptin , cxcl1 , lamina propria , colorectal cancer , medicine , endocrinology , inflammation , leptin receptor , biology , receptor , obesity , epithelium , cancer , chemokine , genetics
Dysregulation of leptin associated with obesity is implicated in obesity‐related colon cancer, but mechanisms are elusive. Increased adiposity and elevated plasma leptin are associated with perturbed metabolism in colon and leptin receptors are expressed on colon epithelium. We hypothesise that obesity increases the sensitivity of the colon to cancer by disrupting leptin‐regulated gene targets within colon tissues. PCR arrays were used to firstly identify leptin responsive genes and secondly to identify responses to leptin challenge in wild‐type mice, or those lacking leptin ( ob / ob ). Leptin‐regulated genes were localised in the colon using in situ hybridisation. IL6 , IL1β and CXCL1 were up‐regulated by leptin and localised to discrete cells in gut epithelium, lamina propria, muscularis and at the peritoneal serosal surface. Leptin regulates pro‐inflammatory genes such as IL6 , IL1β and CXCL1 , and might increase the risk of colon cancer among obese individuals. J. Cell. Physiol. 226: 2123–2130, 2011. © 2010 Wiley‐Liss, Inc.