Premium
Exhaustive exercise increases inflammatory response via toll like receptor‐4 and NF‐κBp65 pathway in rat adipose tissue
Author(s) -
Rosa José C.,
Lira Fábio S.,
Eguchi Ricardo,
Pimentel Gustavo D.,
Venâncio Daniel P.,
Cunha Cláudio A.,
Oyama Lila M.,
De Mello Marco T.,
Seelaender Marília,
Oller do Nascimento Cláudia M.
Publication year - 2011
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22490
Subject(s) - adipose tissue , inflammation , receptor , medicine , endocrinology , proinflammatory cytokine , chemistry , tumor necrosis factor alpha
Cytokines (IL‐6, IL‐10, and TNF‐α) are increased after exhaustive exercise in the retroperitoneal adipose tissue (RPAT) and mesenteric adipose tissue (MEAT). An exhaustive acute exercise protocol induces inflammation in adipose tissue that lasts 6 h after the exercise has ended. It is well‐established that this protocol increases circulating plasma levels of non‐esterified fatty acids (NEFAs) and lipopolysaccharides (LPS), compounds that are important in stimulating signaling via toll like receptor‐4 (TLR‐4) in different type cells. In the present study, we investigated the regulation of TLR‐4 and DNA‐binding of nuclear factor‐κBp65 (NF‐κBp65) in different depots of adipose tissue in rats after exhaustive exercise. Rats were killed by decapitation immediately (E0 group, n = 6), 2 (E2 group, n = 6), and 6 h (E6 group, n = 6) after the exhaustive exercise, which consisted of running on a treadmill (approximately 70% V O2max ) for 50 min and then running at an elevated rate that increased at 1 m/min, until exhaustion. The control group (C group, n = 6) was not subjected to exercise. In RPAT, TLR‐4, MYD‐88, and IkBα increased in the E2 group after exercise. MYD‐88 and TRAF6 remained increased in the E6 group in comparison with the control group. DNA‐binding of NF‐κBp65 was not altered. In MEAT, TLR‐4, MYD‐88, TRAF6, and DNA‐binding of NF‐κBp65 were increased only in the E6 group. In conclusion, we have shown that increases in pro‐inflammatory cytokines in adipose tissue pads after exhaustive exercise may be mediated via TLR‐4 signaling, leading to increases in NF‐κBp65 binding to DNA in MEAT. J. Cell. Physiol. 226: 1604–1607, 2011. © 2010 Wiley‐Liss, Inc.