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Mechanistic insights into reprogramming to induced pluripotency
Author(s) -
Ho Ritchie,
Chronis Constantinos,
Plath Kathrin
Publication year - 2011
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22450
Subject(s) - reprogramming , sox2 , induced pluripotent stem cell , embryonic stem cell , klf4 , biology , microbiology and biotechnology , regenerative medicine , cell potency , transcription factor , homeobox protein nanog , cellular differentiation , stem cell , cell , computational biology , genetics , gene
Abstract Induced pluripotent stem (iPS) cells can be generated from various embryonic and adult cell types upon expression of a set of few transcription factors, most commonly consisting of Oct4, Sox2, cMyc, and Klf4, following a strategy originally published by Takahashi and Yamanaka (Takahashi and Yamanaka, 2006, Cell 126: 663–676). Since iPS cells are molecularly and functionally similar to embryonic stem (ES) cells, they provide a source of patient‐specific pluripotent cells for regenerative medicine and disease modeling, and therefore have generated enormous scientific and public interest. The generation of iPS cells also presents a powerful tool for dissecting mechanisms that stabilize the differentiated state and are required for the establishment of pluripotency. In this review, we discuss our current view of the molecular mechanisms underlying transcription factor‐mediated reprogramming to induced pluripotency. J. Cell. Physiol. 226: 868–878, 2011. © 2010 Wiley‐Liss, Inc.