Premium
RARγ is required for correct deposition and removal of Suz12 and H2A.Z in embryonic stem cells
Author(s) -
Amat Ramon,
Gudas Lorraine J.
Publication year - 2011
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22420
Subject(s) - retinoic acid , epigenetics , embryonic stem cell , histone , biology , stem cell , microbiology and biotechnology , gene , transcription factor , cellular differentiation , cancer research , genetics
Retinoic acid (RA) induces embryonic stem cell differentiation. The effects of RA are mediated by retinoic acid receptors (RARs) that promote epigenetic changes controlling gene transcription. We show here that RARγ, in the absence of the ligand RA, is required for deposition of the histone variant H2A.Z and the polycomb group protein Suz12 at RA target genes, and that in embryonic stem cells both RARγ and Suz12 exist in a multi‐protein complex in the absence of ligand. Addition of RA causes removal of H2A.Z and Suz12 from RARγ target genes when the genes are transcriptionally activated. J. Cell. Physiol. 226: 293–298, 2011. © 2010 Wiley‐Liss, Inc.