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Artefactual effects of oxygen on cell culture models of cellular senescence and stem cell biology
Author(s) -
Toussaint Olivier,
Weemaels Geoffroy,
DebacqChainiaux Florence,
ScharffetterKochanek Karin,
Wlaschek Meinhard
Publication year - 2011
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22416
Subject(s) - oxygen tension , senescence , in vivo , cell culture , biochemical engineering , cell , biology , microbiology and biotechnology , oxygen , computational biology , chemistry , biochemistry , genetics , engineering , organic chemistry
Abstract In life sciences, modelling of the in vivo conditions using in vitro models is an important tool to generate knowledge. Although aerobic organisms including mammals depend on accurate oxygen tension, mimicking physiological conditions in cell culture experiments is not very common. Due to the need for simple technical and experimental design, the requirement for simulating the in vivo oxygen tension parameters has been neglected over long time. Fortunately, due to increasing knowledge in recent years the attention has shifted towards this scientific demand. In this short review, we summarize data substantiating the necessity to adequately mimic physiological oxygen tension using cell culture models in life science research. J. Cell. Physiol. 226: 315–321, 2011. © 2010 Wiley‐Liss, Inc.