Premium
Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva
Author(s) -
Tang Liang,
Li Nan,
Xie Han,
Jin Yan
Publication year - 2011
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22405
Subject(s) - mesenchymal stem cell , transplantation , microbiology and biotechnology , in vivo , stem cell , cell , lamina propria , immunology , biology , medicine , pathology , epithelium , surgery , biochemistry
Human gingiva plays an important role in the maintenance of oral health and shows unique fetal‐like scarless healing process after wounding. Here we isolate and characterize mesenchymal stem cells from human normal and hyperplastic gingival tissues (N‐GMSC and H‐GMSC, respectively). Immunocytochemical staining indicated that gingival lamina propria contained Stro‐1 and SSEA‐4 positive cells, implying existence of putative gingival MSC. Under attachment‐based isolating and culturing condition, gingival MSC displayed highly clonogenic and long‐term proliferative capability. By using single colony isolation and expansion approaches, we found both N‐GMSC and H‐GMSC possessed self‐renewal and multipotent differentiation properties. N‐GMSC and H‐GMSC showed distinct immunoregulatory functions in a murine skin allograft setting via up‐regulation of putative systemic regulatory T cells (Tregs). N‐GMSC and H‐GMSC were capable of regenerating collagenous tissue following in vivo transplantation, in which H‐GMSC exhibited more robust regenerative capability. These findings suggest that gingival tissue contains tissue‐specific mesenchymal stem cell population and is an ideal resource for immunoregulatory therapy due to its substantial availability and accessibility. In addition, gingival MSC over‐activation may contribute to gingival hyperplastic phenotype. J. Cell. Physiol. 226: 832–842, 2011. © 2010 Wiley‐Liss, Inc.