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Expression of Micro‐RNA‐145 is regulated by a highly conserved genomic sequence 3′ to the pre‐miR
Author(s) -
La Rocca Gaspare,
Shi Bin,
SeppLorenzino Laura,
Baserga Renato
Publication year - 2011
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22368
Subject(s) - sequence (biology) , rna , microrna , computational biology , biology , conserved sequence , genetics , microbiology and biotechnology , gene , base sequence
Micro‐RNA‐145 (miR145), a tumor suppressor miR, dramatically inhibits growth of cancer cells in culture and plays a significant role in human stem cells differentiation. We have isolated a human genomic sequence of 864 bp comprising the pre‐miR and its flanking sequences. The cloned miR145 genomic sequence expresses a mature miR145 in transfected cells. We show here that flanking sequences on either side of the pre‐miR sequence can modulate its expression levels. Surprisingly, a highly conserved sequence 3′ to the pre‐miR plays a crucial role in miR145 expression. J. Cell. Physiol. 226: 602–607, 2011. © 2010 Wiley‐Liss, Inc.
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