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Competitiveness for the niche and mutual dependence of the germline and somatic stem cells in the Drosophila testis are regulated by the JAK/STAT signaling
Author(s) -
Singh Shree Ram,
Zheng Zhiyu,
Wang Hong,
Oh SuWan,
Chen Xiu,
Hou Steven X.
Publication year - 2010
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22073
Subject(s) - biology , jak stat signaling pathway , janus kinase , stat , stem cell , microbiology and biotechnology , germline , somatic cell , stat protein , signal transduction , transcription factor , stat4 , stat3 , genetics , gene , tyrosine kinase
In many tissues, two or more types of stem cells share a niche, and how the stem cells coordinate their self‐renewal and differentiation is poorly understood. In the Drosophila testis, germ line stem cells (GSCs) and somatic cyst progenitor cells (CPCs) contact each other and share a niche (the hub). The hub expresses a growth factor unpaired (Upd) that activates the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway in GSCs to regulate the stem cell self‐renewal. Here, we demonstrate that the JAK/STAT signaling also regulates CPCs self‐renewal. We also show that a negative regulator, the suppressor of cytokine signaling 36E (SOCS36E), suppresses JAK/STAT signaling in somatic cells, preventing them from out‐competing the GSCs. Furthermore, through selectively manipulating the JAK/STAT signaling level in either CPCs or GSCs, we demonstrate that the somatic JAK/STAT signaling is essential for self‐renewal and maintenance of both CPCs and GSCs. These data suggest that a single JAK/STAT signal from the niche orchestrate the competitive and dependent co‐existence of GSCs and CPCs in the Drosophila testis niche. J. Cell. Physiol. 223: 500–510, 2010. © 2010 Wiley‐Liss, Inc.