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Heat shock but not cold shock leads to disturbed intracellular zinc homeostasis
Author(s) -
Pirev Elvis,
Ince Yasemin,
Sies Helmut,
Kröncke Klaus D.
Publication year - 2010
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.22016
Subject(s) - intracellular , homeostasis , zinc , heat shock , shock (circulatory) , heat shock protein , microbiology and biotechnology , metallothionein , biophysics , chemistry , biology , biochemistry , medicine , organic chemistry , gene
The heat shock response is a highly conserved process essential for surviving environmental stress, including extremes of temperature. To investigate whether heat shock has an impact on intracellular Zn 2+ homeostasis, cells were subjected to heat shock, and subsequently the intracellular free zinc concentration was investigated. Sublethal heat shock induced a temperature‐dependent and transient intracellular Zn 2+ release that was repeatable after 24 h. The free zinc was localized in round‐shaped nuclear bodies identified as nucleoli. Metallothionein, the main cellular zinc storing protein, was found to be not functionally essential for this heat‐shock‐induced effect. No significant oxidative stress within the cells was detected after heat shock. Cold shock and subsequent rewarming did not result in disturbed intracellular zinc homeostasis. These results show that heat shock and cold shock differ with respect to intracellular Zn 2+ release. A role for zinc as signaling ion during fever is conceivable. J. Cell. Physiol. 223: 103–109, 2010. © 2009 Wiley‐Liss, Inc.