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Heterogeneity of non‐cycling and cycling synchronized murine hematopoietic stem/progenitor cells
Author(s) -
Colvin Gerald A.,
Berz David,
Liu Liansheng,
Dooner Mark S.,
Dooner Gerri,
Pascual Sheila,
Chung Samuel,
Sui Yunxia,
Quesenberry Peter J.
Publication year - 2010
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.21918
Subject(s) - stem cell , biology , haematopoiesis , progenitor cell , microbiology and biotechnology , population , cell cycle , immunology , genetics , cell , demography , sociology
Abstract Purified long‐term multilineage repopulating marrow stem cells have been considered to be homogenous, but functionally these cells are heterogeneous. Many investigators urge clonal studies to define stem cells but, if stem cells are truly heterogeneous, clonal studies can only define heterogeneity. We have determined the colony growth and differentiation of individual lineage negative, rhodamine low, Hoechst low (LRH) stem cells at various times in cytokine culture, corresponding to specific cell cycle stages. These highly purified and cycle synchronized (98% in S phase at 40 h of culture) stem cells were exposed to two cytokine cocktails for 0, 18, 32, or 40 h and clonal differentiation assessed 14 days later. Total heterogeneity as to gross colony morphology and differentiation stage was demonstrated. This heterogeneity showed patterns of differentiation at different cycle times. These data hearken to previous suggestions that stem cells might be similar to radioactive isotopes; decay rate of a population of radioisotopes being highly predictable, while the decay of individual nuclei is heterogeneous and unpredictable (Till et al., 1964). Marrow stem cells may be most adequately defined on a population basis; stem cells existing in a continuum of reversible change rather than a hierarchy. J. Cell. Physiol. 222:57–65, 2010. © 2009 Wiley‐Liss, Inc.