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Generation of a human immunodeficiency virus type 1 chronically infected monkey B cell line expressing low levels of endogenous TRIM5α
Author(s) -
Ridolfi Barbara,
Catone Stefania,
Sgarbanti Marco,
Sernicola Leonardo,
Battistini Angela,
Parolin Cristina,
Titti Fausto,
Borsetti Alessandra
Publication year - 2009
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.21914
Subject(s) - virology , biology , virus , peripheral blood mononuclear cell , cell culture , infectivity , viral replication , cell , pathogenesis , microbiology and biotechnology , in vitro , immunology , genetics
Abstract Several innate cellular antiviral factors exist in mammalian cells that prevent the replication of retroviruses. Among them, the tripartite motif protein (TRIM)5α has been shown to block human immunodeficiency virus type 1 (HIV‐1) infection in several types of Old World monkey cells. Here we report a novel HIV‐1 chronically infected monkey B cell line, F6/HIV‐1, characterized by very low levels of TRIM5α expression that allows HIV‐1 to overcome the restriction. Virus produced by F6/HIV‐1 cells fails to infect monkey cells but retains the ability to infect human peripheral blood mononuclear cells (PBMCs) and T cell lines, although with a reduced infectivity compared to the input virus. Ultrastructural analyses revealed the presence of budding virions at the F6/HIV‐1 cells plasma membrane characterized by a typical conical core shell. To our knowledge F6/HIV‐1 is the first monkey cell line chronically infected by HIV‐1 and able to release infectious particles thus representing a useful tool to gain further insights into the molecular mechanisms of HIV‐1 pathogenesis. J. Cell. Physiol. 221: 760–765, 2009. © 2009 Wiley‐Liss, Inc.