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Alterations in the temporal expression and function of cadherin‐7 inhibit cell migration and condensation during chondrogenesis of chick limb mesenchymal cells in vitro
Author(s) -
Kim Dongkyun,
Kang ShinSung,
Jin EunJung
Publication year - 2009
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.21840
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , chondrogenesis , cell migration , cadherin , motility , progenitor cell , cell , chemistry , gene knockdown , biology , cell culture , stem cell , biochemistry , genetics
Abstract Endochondral bone formation requires a complex interplay among immature mesenchymal progenitor cells to form the cartilaginous anlagen, which involves migration, aggregation and condensation. Even though condensation of chondrogenic progenitors is an essential step in this process, its mechanism(s) has not been well studied. Here, we show that cadherin‐7 plays a central role in cellular condensation by modulating cell motility and migration. In this study, many mesenchymal cells failed to migrate, and precartilage condensation was inhibited, after knockdown of endogenous cadherin‐7 levels. Exposure of mesenchymal cells to SB203580 (a specific inhibitor of p38MAPK), LiCl (an inhibitor of GSK‐3β) or overexpression of β‐catenin resulted in inhibition of cadherin‐7 levels and, subsequently, suppression of cell migration. Collectively, our results suggest that cadherin‐7 controls cell migration in chick limb bud mesenchymal cells, and that p38MAPK and GSK signals are responsible for regulating cadherin‐7‐mediated cell migration. J. Cell. Physiol. 221: 161–170, 2009. © 2009 Wiley‐Liss, Inc