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Lack of lymphatic vessel phenotype in LYVE‐1/CD44 double knockout mice
Author(s) -
Luong Mai X.,
Tam Joshua,
Lin Qingcong,
Hagendoorn Jeroen,
Moore Kathryn J.,
Padera Timothy P.,
Seed Brian,
Fukumura Dai,
Kucherlapati Raju,
Jain Rakesh K.
Publication year - 2009
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.21686
Subject(s) - lymphatic system , lymphatic vessel , knockout mouse , lymphatic endothelium , inflammation , pathology , cd44 , biology , lymphangiogenesis , intravital microscopy , receptor , immunology , in vivo , medicine , cell , metastasis , cancer , biochemistry , genetics , microbiology and biotechnology
Lymphatic vessels play a key role in maintaining tissue‐fluid homeostasis, immune surveillance and metastasis. The hyaluronan receptor, LYVE‐1, is widely used as a molecular marker for adult and embryonic lymphatic endothelium, but its physiological functions have not yet been established in vivo. In agreement with a recent report, LYVE‐1 −/− mice, which are healthy and fertile, do not display any defects related to congenital abnormalities of the lymphatic system. One hypothesis for the absence of a phenotype in LYVE‐1 null mice is that other hyaluronan receptors, such as CD44, may compensate for LYVE‐1. To test this hypothesis, we created LYVE‐1/CD44 double knockout mice with appropriate littermate controls. Lymphatic vessel structure and function, as determined by histological methods and intravital microscopy, show that LYVE‐1 −/− , CD44 −/− and LYVE‐1 −/− /CD44 −/− mice are indistinguishable from wild‐type mice under normal conditions. Furthermore, resolution of carrageenan‐induced paw edema is comparable in all genotypes. However, LYVE‐1 −/− /CD44 −/− mice exhibit increased edema formation in a carrageenan‐induced paw inflammation model compared to wild‐type mice, but not to LYVE −/− or CD44 −/− mice. These data suggest that LYVE‐1 and CD44 are not required for the formation or function of lymphatics, but do not rule out a role for LYVE‐1 in inflammation. J. Cell. Physiol. 219: 430–437, 2009. © 2009 Wiley‐Liss, Inc.