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Phorbol ester‐induced podosomes in normal human bronchial epithelial cells
Author(s) -
Xiao Helan,
Eves Rob,
Yeh Christina,
Kan Wayne,
Xu Feng,
Mak Alan S.,
Liu Mingyao
Publication year - 2009
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.21609
Subject(s) - podosome , microbiology and biotechnology , cytoskeleton , cell migration , actin , epithelium , biology , fibronectin , cell , chemistry , extracellular matrix , biochemistry , genetics
Spreading and migration of the basal cells neighboring a wound is essential for airway epithelial repair. To gain insight into the molecular mechanisms that govern these cellular processes, we asked whether normal human airway epithelial cells can form podosomes, a cellular structure discovered from cancer and mesenchymal cells that controls migration and invasion. Herein, we report that phorbol‐12, 13‐dibutyrate (PDBu), a protein kinase C activator, induced reorganization of cytoskeletal structure in primary normal human bronchial epithelial cells, and in normal human airway epithelial BEAS2B cells. Z‐stack scanning confocal microscopy showed that PDBu‐induced podosome‐like structures contain actin‐rich columns that arise from the ventral surface of the cell, and also revealed the presence of circular ruffles/waves at the dorsal cell surface. The molecular components of these cytoskeletal structures were determined with immunofluorescent staining. Using in situ zymography, we demonstrated that PDBu‐induced podosomes were capable of degrading fibronectin‐gelatin‐sucrose matrix. PDBu also increased epithelial cell invasion across Transwell chamber. Podosomes and circular dorsal ruffles may be important for epithelial cell migration and invasion, thus contributing to respiratory epithelial repair and regeneration. J. Cell. Physiol. 218: 366–375, 2009. © 2008 Wiley‐Liss, Inc.