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Systemically transplanted bone marrow stromal cells contributing to bone tissue regeneration
Author(s) -
Li S.,
Tu Q.,
Zhang J.,
Stein G.,
Lian J.,
Yang P.S.,
Chen J.
Publication year - 2008
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.21302
Subject(s) - calvaria , stromal cell , bone marrow , regeneration (biology) , luciferase , green fluorescent protein , pathology , transplantation , immunohistochemistry , progenitor cell , andrology , chemistry , anatomy , microbiology and biotechnology , stem cell , biology , medicine , surgery , transfection , in vitro , gene , biochemistry
Bone marrow stromal cells (BMSCs) are a rich source of osteogenic progenitor cells. A fundamental question is whether systemically transplanted BMSCs participate in bone regeneration. Luciferase and GFP double‐labeled BMSCs were transplanted into irradiated mice. Five weeks after transplantation, artificial bone wounds were created in the mandibles and calvaria of the recipients. Animals were sacrificed at weeks 2, 4, and 6 after surgery and the expressions of luciferase and GFP were determined using Xenogen IVIS Imaging System, immunohistochemical staining and RT‐PCR. The results demonstrated that transplanted BMSCs can be detected in wound sites as early as 2 weeks and lasted the whole experimental period. Luciferase expression peaked at 2 weeks after surgery and decreased thereafter, exhibiting a similar expression pattern as that of BSP, while GFP expression was relatively stable during the experimental period. In conclusion, BMSCs can migrate to bone wound sites and participate in bone regeneration in orocraniofacial region. J. Cell. Physiol. 215: 204–209, 2008. © 2007 Wiley‐Liss, Inc.