Premium
The MAP kinase JNK‐1 of Caenorhabditis elegans : Location, activation, and influences over temperature‐dependent insulin‐like signaling, stress responses, and fitness
Author(s) -
Wolf Marc,
Nunes Frank,
Henkel Arne,
Heinick Alexander,
Paul Rüdiger J.
Publication year - 2008
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.21269
Subject(s) - caenorhabditis elegans , microbiology and biotechnology , kinase , mapk/erk pathway , mitogen activated protein kinase , insulin receptor , phosphorylation , mutant , biology , signal transduction , insulin , biochemistry , gene , endocrinology , insulin resistance
The mitogen‐activated protein kinase (MAPK) pathways and insulin‐like signaling play pivotal roles in cellular stress response. Using an anti‐phospho‐SAPK/JNK antibody and a daf‐16 ::GFP‐based reporter assay, the present study shows in Caenorhabditis elegans that ambient temperature (1–37°C) specifically influences the activation (phosphorylation) of the MAP kinase JNK‐1 as well as the nuclear translocation of DAF‐16, the main downstream target of insulin‐like signaling. Activated JNK‐1 was detected only in neuronal cells, and JNK‐1 was found to be controlled by the MAPK JKK‐1 under heat stress. Comparative analyses on the wildtype and a jnk‐1 deletion mutant revealed a promoting influence of JNK‐1 on both nuclear DAF‐16 translocations and DAF‐16 target gene ( superoxide dismutase 3 , sod‐3 ) expressions within peripheral, non‐neuronal tissue. Consequently, the mutant exhibited a reduced thermal tolerance and reproductive fitness at higher temperatures. These results provide evidence of indirect interactions between neuronal MAPK and peripheral insulin‐like signaling in response to environmental stimuli (temperature, H 2 O 2 ). J. Cell. Physiol. 214: 721–729, 2008. © 2007 Wiley‐Liss, Inc.