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Paxillin's LD4 motif interacts with bcl‐2
Author(s) -
Sheibani Nader,
Tang Yixin,
Sorenson Christine M.
Publication year - 2008
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.21256
Subject(s) - paxillin , microbiology and biotechnology , integrin , focal adhesion , rgd motif , biology , signal transducing adaptor protein , cell adhesion , signal transduction , receptor , cell , biochemistry
bcl‐2 is the founding member of a family of proteins which influences cell survival in response to a variety of stimuli including those from growth factor receptors and integrins. However, how these activities are coordinated through bcl‐2 requires further investigation. bcl‐2 interacts with paxillin, potentially linking cell survival and cell adhesive pathways. Paxillin is an adapter protein implicated in growth factor and integrin‐mediated signal transduction pathways. Previous work in this laboratory demonstrated that loss of bcl‐2 affects cell adhesion and migration characteristics of renal epithelial cells, perhaps through disruption of its interaction with paxillin. Here studies were performed to determine the bcl‐2 binding motif in paxillin. The amino‐terminal portion of paxillin, specifically its LD4 motif, was found to associate with bcl‐2. However, the amino‐terminal portion of paxillin with the LD4 domain deleted did not associate with bcl‐2. The corresponding LD motif in other paxillin family members, Hic‐5 and leupaxin, did not associate with bcl‐2. Mutations in paxillin's LD4 motif made to mimic Hic‐5 and leupaxin LD4‐like motifs (E 268 → R or S 272 → H) abolished its association with bcl‐2. Incubation of embryonic kidneys with paxillin's LD4 motif disrupted ureteric bud branching and morphogenesis, while incubation with the comparable Hic‐5 LD motif did not significantly affect morphogenesis. These data suggest that paxillin's association with bcl‐2 plays a unique role during kidney development that other paxillin family members may not be able to fulfill. J. Cell. Physiol. 214: 655–661, 2008. © 2007 Wiley‐Liss, Inc.