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The chromatin of differentiating erythroblasts is cleaved into large size fragments independent of caspase activated DNase and apoptosis inducing factor
Author(s) -
Hristoskova Sashka,
Holzgreve Wolfgang,
Hahn Sinuhe,
Rusterholz Corinne
Publication year - 2007
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.21125
Subject(s) - chromatin , apoptotic dna fragmentation , microbiology and biotechnology , dna fragmentation , hypersensitive site , erythroblast , biology , fragmentation (computing) , apoptosis , nuclease , cleavage (geology) , dna , deoxyribonuclease i , programmed cell death , stem cell , haematopoiesis , genetics , fracture (geology) , base sequence , ecology , paleontology
Erythroblast cell differentiation involves self‐controlled and limited nuclear proteolysis prior nucleus loss. Early evidence suggests that apoptotic‐like pathways are activated during this process. The chromatin of developing erythroblasts becomes fragmented in vivo, however, the exact mechanisms and molecules involved remain elusive. In this study, erythroblasts were differentiated in culture from CD34‐enriched umbilical cord blood progenitor cells and the characteristics of DNA fragmentation were examined. This analysis shows that the chromatin of differentiating erythroblasts is cleaved into discrete fragments of 50–200 kb. This process most likely involves one or several endonucleases as we detect in vivo double strand DNA cleavage. However, major players of the apoptotic DNA degradation, caspase activated DNase and apoptosis inducing factor, are not activated in these cells. Therefore, our data suggests that erythroblast chromatin degradation may involve enzymes distinct form those active in apoptotic cells. J. Cell. Physiol. 213: 490–494, 2007. © 2007 Wiley‐Liss, Inc.