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AKT and MAPK signaling in KGF‐treated and UVB‐exposed human epidermal cells
Author(s) -
Lotti Lavinia Vittoria,
Rotolo Sabrina,
Francescangeli Federica,
Frati Luigi,
Torrisi Maria Rosaria,
Marchese Cinzia
Publication year - 2007
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.21056
Subject(s) - keratinocyte growth factor , protein kinase b , mapk/erk pathway , microbiology and biotechnology , keratinocyte , signal transduction , cell growth , cellular differentiation , downregulation and upregulation , biology , pi3k/akt/mtor pathway , chemistry , growth factor , receptor , cell culture , biochemistry , genetics , gene
Regulation of proliferation and differentiation in keratinocyte is a complex and dynamic process that involves activation of multiple signaling pathways triggered by different growth factors. Keratinocyte growth factor (KGF) is not only a potent mitogen, but differently from other growth factors, is a potent inducer of differentiation. The MAP kinase and AKT pathways are involved in proliferation and differentiation of many cell types including keratinocytes. We investigated here the role of KGF in modulating AKT and MAPK activity during differentiation of human keratinocytes. Our results show that the mechanisms of action of KGF are dose‐dependent and that a sustained activation of the MAPK signaling cascade causes a negative regulation of AKT. We also demostrated increasing expression of KGFR substrates, such as PAK4 during keratinocyte differentiation parallel to the receptor upregulation. J. Cell. Physiol. 212:633–642, 2007. © 2007 Wiley‐Liss, Inc.