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P2Y 2 receptors induced cell surface redistribution of α v integrin is required for activation of ERK 1/2 in U937 cells
Author(s) -
Chortaliya E.,
Chevres Migdalia,
SantosBerrios Cynthia,
Orellano Elsie A.,
Erb Laurie,
González Fernando A.
Publication year - 2007
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.20946
Subject(s) - redistribution (election) , receptor , integrin , microbiology and biotechnology , mapk/erk pathway , chemistry , u937 cell , cell , cell surface receptor , biophysics , biology , biochemistry , signal transduction , apoptosis , politics , political science , law
Nucleotides released from cells due to stress, injury or inflammation, induce mitogenic effects in monocytes via activation of P2Y 2 nucleotide receptors (P2Y 2 Rs). Here we show that P2Y 2 nucleotide receptors in U937 monocytic cells regulate the activation of extracellular signal‐regulated kinases 1 and 2 (ERK 1/2) by inducing the clustering of α v integrins. The activation of phosphatidylinositol 3‐kinase by P2Y 2 R ligands was required for α v clustering, suggesting a means whereby two different classes of receptors communicate to induce mitogenic responses in monocytic cells. P2Y 2 R‐induced α v clustering was also associated with a flattened phenotype of the U937 cells, consistent with the role of the P2Y 2 R in regulating early events in cell migration. J. Cell. Physiol. 211: 410–422, 2007. © 2006 Wiley‐Liss, Inc.