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Activation of human endothelial cells by tumor necrosis factor‐α results in profound changes in the expression of glycosylation‐related genes
Author(s) -
GarcíaVallejo Juan J.,
Van Dijk Willem,
Van Het Hof Bert,
Van Die Irma,
Engelse Marten A.,
Van Hinsbergh Victor W.M.,
Gringhuis Sonja I.
Publication year - 2006
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.20458
Subject(s) - biology , glycosylation , microbiology and biotechnology , endothelial stem cell , tumor necrosis factor alpha , human umbilical vein endothelial cell , umbilical vein , glycan , endothelium , cell , inflammation , immunology , biochemistry , glycoprotein , in vitro , genetics
The endothelium plays a central role in the logistics of the immune system by allowing the selective transmigration of leukocytes, as well as the maintenance of the circulation and coagulation homeostasis. Evidence is increasing that the carbohydrate composition of the endothelial cell surface is critical for the cells to exert their physiological function. The major aim of this study is to unravel the mechanisms underlying the expression of carbohydrate structures by endothelial cells, which are involved in leukocyte adhesion and migration. Using quantitative real‐time PCR, the expression profile of a selected group of 74 glycosylation‐related genes has been determined in human umbilical vein endothelial cells (HUVEC) and human foreskin microvascular endothelial cells (FMVEC) under control and TNFα‐induced conditions. The set of genes comprised 59 glycosyltransferases, 6 mannosidases and 9 sulfotransferases. In parallel, the overall cell surface glycan profile has been assessed by the use of glycan‐specific lectins and monoclonal antibodies. The results demonstrate that HUVEC and FMVEC differ substantially in the expression of glycosylation‐related genes and, accordingly, also in the presence of different glycan epitopes on the cell membrane. Induction of an inflamed phenotype of the cells by treatment with TNFα differentially modulates a set of these genes in HUVEC and FMVEC resulting in a change in the cell membrane associated glycans that are of importance in inflammation‐related endothelial cell‐surface processes. © 2005 Wiley‐Liss, Inc.