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EEDA: A protein associated with an early stage of stratified epithelial differentiation
Author(s) -
Sun Lijie,
Ryan David G.,
Zhou Mingyuan,
Sun TungTien,
Lavker Robert M.
Publication year - 2006
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.20433
Subject(s) - biology , keratin , epithelium , suppression subtractive hybridization , epidermis (zoology) , cellular differentiation , basal (medicine) , microbiology and biotechnology , stem cell , in situ hybridization , corneal epithelium , embryonic stem cell , messenger rna , anatomy , gene , endocrinology , genetics , cdna library , insulin
Using suppressive subtractive hybridization, we have identified a novel gene, which we named early epithelial differentiation associated (EEDA), which is uniquely associated with an early stage of stratified epithelial differentiation. In epidermis, esophageal epithelium, and tongue epithelium, EEDA mRNA, and antigen was abundant in suprabasal cells, but was barely detectable in more differentiated cells. Consistent with the limbal location of corneal epithelial stem cells, EEDA was expressed in basal corneal epithelial cells that are out of the stem cell compartment, as well as the suprabasal corneal epithelial cells. The strongest EEDA expression occurred in suprabasal precortical cells of mouse, bovine, and human anagen follicles. Developmental studies showed that the appearance of EEDA in embryonic mouse epidermis (E 15.5) coincided with morphological keratinization. Interestingly, EEDA expression is turned off when epithelia were perturbed by wounding and by cultivation under both low and high Ca 2+ conditions. Our results indicate that EEDA is involved in the early stages of normal epithelial differentiation, and that EEDA is important for the “normal” differentiation pathway in a wide range of stratified epithelia. © 2005 Wiley‐Liss, Inc.