z-logo
Premium
Changes in cell shape and anchorage in relation to the restriction point
Author(s) -
Martinsson HannaStina,
Zickert Peter,
Starborg Maria,
Larsson Olle,
Zetterberg Anders
Publication year - 2005
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.20204
Subject(s) - mitosis , growth factor , cell cycle , cell , biology , microbiology and biotechnology , cell growth , epidermal growth factor , restriction point , retinoblastoma protein , chemistry , biophysics , receptor , biochemistry , cell cycle progression
The restriction point (R) separates the G 1 phase of continuously cycling cells into two functionally different parts. The first part, G 1 ‐pm, represents the growth factor dependent post‐mitotic interval from mitosis to R, which is of constant length (3–4 h). The second part, G 1 ‐ps, represents the growth factor independent, pre‐S phase interval of G 1 that lasts from R to S and that varies in time from 1 to 10 h. G 1 ‐pm cells rapidly exit (within 1 h) from the cell cycle and enter G 0 as a response to serum withdrawal. The finding that R occurs at a set time after mitosis indicates that R may be related to the metabolic and/or structural changes that the cell underwent during the previous mitosis. We have recently shown that phosphorylation of the retinoblastoma tumor suppressor protein (pRb) is not the molecular mechanism behind R, as has been suggested previously. Here, we present an alternative explanation for R. In the present study, we applied a single cell approach using time‐lapse analysis, which revealed that upon serum starvation the G 1 ‐pm cells rapidly underwent a transient change in cell shape from flat to spherical before exiting to G 0 . Platelet derived growth factor (PDGF) counteracted this change in shape and also prevented exit to G 0 to the same extent. Furthermore epidermal growth factor (EGF) and insulin like growth factor (IGF‐1), which only partially counteracted this change, only partially counteracts exit to G 0 . These data clearly indicate a direct link between change in cell shape and exit to G 0 in G 1 ‐cells that have not passed R. © 2004 Wiley‐Liss, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here