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Circadian variations of bone marrow engraftability
Author(s) -
D'Hondt Lionel,
McAuliffe Christina,
Damon Jeffrey,
Reilly Judith,
Carlson Jane,
Dooner Mark,
Colvin Gerald,
Lambert JeanFrançois,
Hsieh ChungCheng,
Habibian Houri,
Stencel Kimberly,
Quesenberry Peter J.
Publication year - 2004
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.20032
Subject(s) - circadian rhythm , bone marrow , halo , spleen , congenic , haematopoiesis , biology , progenitor cell , bone marrow transplant , immunology , medicine , endocrinology , stem cell , bone marrow transplantation , microbiology and biotechnology , genetics , physics , quantum mechanics , galaxy , gene
Circadian rhythms exist for hematopoiesis, but little is known about circadian variation of bone marrow engraftability and host “acceptability”. Using a B6.SJL to C57BL/6J congenic transplant model, we chose 3‐times with light on: Hours After Light Onset (HALO) 4, 8, and 12 and 3‐times with light off: HALO 16, 20, and 24. The mice were conditioned on a 12‐h light/dark cycles. Recipient mice (100 cGy) received 40 million cells. We demonstrated a significant variation of bone marrow engraftability into bone marrow, spleen, and thymus when donor animals were subjected to changes in their light/dark cycles. Two statistically significant nadirs in all three organs were observed at HALO 8 and 24 in experiments carried out in July, while an identical set of experiments in February analyzing engraftment in marrow and spleen showed nadirs at HALO 8, but not at HALO 24. Marrow progenitors from the July experiments showed nadirs at HALO 12 and 24. The percentage of progenitors in S phase peaked at HALO 8 and 24. Interestingly, there were no changes in the ability of host to accept grafts with changes in the light/dark cycles of host animals. Circadian variations of bone marrow engraftability are important and should be considered in bone marrow transplant strategies. © 2004 Wiley‐Liss, Inc.