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Exogenous nitric oxide elicits chemotaxis of neutrophils in vitro
Author(s) -
Beauvais Francis,
Michel Laurence,
Dubertret Louis
Publication year - 1995
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041650319
Subject(s) - chemotaxis , nitric oxide , in vitro , chemistry , microbiology and biotechnology , nitric oxide synthase , biology , biophysics , biochemistry , receptor , organic chemistry
Nitric oxide (NO) has been shown to be both an intercellular and intracellular messenger. We propose here that exogenous NO induces chemotactic locomotion of human neutrophils. Indeed, when human neutrophils were placed in a gradient of a nitric oxide donor (S‐nitroso‐N‐acetylpenicillamine; SNAP), a directed locomotion was induced, as evidenced by experiments of chemotaxis under agarose. Degraded SNAP (i.e., SNAP solution which had previously released NO) did not induce directed locomotion. Moreover, oxyhemoglobin, a scavenger of free NO, suppressed the chemotactic effect of SNAP, whereas LY‐83583, a soluble guanylate cyclase inhibitor, inhibited the SNAP‐mediated chemotaxis in a dose‐response manner. Other unrelated NO donors, SIN‐1 and S‐nitroso‐cysteine—a natural S‐nitroso‐compound, also induced a directed locomotion of neutrophils. Taken together, these in vitro experiments indicate that exogenous NO could mediate the chemotaxis of neutrophils and thus suggest that NO could contribute to neutrophil recruitment in vivo. © 1995 Wiley‐Liss Inc.