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Secretion of plasminogen activator and lysosomal enzymes from mouse skeletal muscle: Effect of denervation
Author(s) -
Von Steyern Fredrik Vult,
Josefsson JanOwe
Publication year - 1995
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041640313
Subject(s) - secretion , brefeldin a , plasminogen activator , medicine , skeletal muscle , activator (genetics) , endocrinology , denervation , enzyme , chemistry , extracellular , biology , biochemistry , cell , receptor , golgi apparatus
Abstract Levels of hydrolytic enzymes increase in skeletal muscle after denervation and their activities in the extracellular matrix appear to be important for interaction between muscle and nerve. Using enzymatic assays for b̃‐glucuronidase, b̃‐galactosidase, and plasminogen activator, we show that secretion of these enzymes from mouse skeletal muscle increases after denervation and that drugs interfering with the secretory pathway or the reuptake of enzymes modulate this release. Thus, brefeldin A inhibited secretion of plasminogen activator activity and mannan increased secreted amounts of b̃‐glucuronidase, but not of b̃‐galactosidase, in denervated muscle. In innervated muscle, brefeldin A decreased secreted activity of plasminogen activator, but mannan had no effect on secretion of either b̃‐glucuronidase or b̃‐galactosidase. Furthermore, secretion of plasminogen activator was temperature dependent. These observations, together with previous studies, suggest that secretion of hydrolytic enzymes from adult skeletal muscle may be of physiological significance in nerve‐muscle communication. © 1995 Wiley‐Liss, Inc.