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Bone marrow stromal cells selectively stimulate the rapid expansion of lineage‐restricted myeloid progenitors
Author(s) -
Kameoka JunIchi,
Yanai Nobuaki,
Obinata Masuo
Publication year - 1995
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041640108
Subject(s) - stromal cell , haematopoiesis , bone marrow , progenitor cell , myeloid , monocyte , microbiology and biotechnology , lymph node stromal cell , granulocyte , biology , immunology , stem cell , cancer research
Bone marrow stromal cells serve hematopoietic microenvironments where different blood cells are controlled in their growth and differentiation. To characterize functions of stromal cells, 33 bone marrow stromal cells including preadipocytes, endothelial cells, and fibroblasts were established from transgenic mice harboring temperature‐sensitive SV40 T‐antigen gene and their selective stimulatory abilities to support large colony formation of lineage‐specific hematopoietic progenitor cells (erythroid, monocyte/macrophage, granulocyte, and monocyte‐granulocyte) were examined. Among established stromal cells, 27 clones showed erythropoietic stimulatory activity in the presence of erythropoietin. On myeloid progenitors, the stromal cells showed lineage‐restricted stimulatory activity and a reciprocal relationship was observed between granulocyte formation and macrophage formation, but these activities were not dependent on the amount of produced colony‐stimulating factors (CSFs). Our present study with many stromal cells established from bone marrow indicated that each stromal cell in the bone marrow may provide the preferable microenvironment for a rapid expansion of the lineage‐restricted progenitor cells in combination with CSFs. © 1995 Wiley‐Liss, Inc.