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Ascorbic acid stimulates barrier function of cultured endothelial cell monolayer
Author(s) -
Utoguchi Naoki,
Ikeda Kenji,
Saeki Kazuhiko,
Oka Naomi,
Mizuguchi Hiroyuki,
Kubo Kazuyosi,
Nakagawa Shinsaku,
Mayumi Tadanori
Publication year - 1995
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041630219
Subject(s) - ascorbic acid , umbilical vein , endothelial stem cell , dithiothreitol , permeability (electromagnetism) , human umbilical vein endothelial cell , biochemistry , chemistry , monolayer , in vitro , biophysics , biology , enzyme , food science , membrane
Abstract The macromolecular permeability of cultured bovine aortic, bovine venous, and human umbilical vein endothelial cell monolayers was decreased significantly in culture medium containing L‐ascorbic acid (Asc Acid; 0.01–0.1 mM) and L‐ascorbic acid 2‐phosphate (Asc 2‐P). Dithiothreitol, which shows reducing activity equivalent to that of Asc Acid, did not affect endothelial permeability. Asc Acid induced a sixfold increase in collagen synthesis by the endothelial cells. The coexistence of L‐azetidine 2‐carboxylic acid, an inhibitor of collagen synthesis, attenuated the effect of Asc 2‐P in a dose‐dependent manner. Another collagen synthesis inhibitor, ethyl‐3,4‐dihydroxybenzoate, also inhibited collagen synthesis and increased endothelial permeability. The decrease in permeability of the endothelial monolayer was dependent on a reduction of the permeability coefficient of the endothelial monolayer. These findings indicate that endothelial barrier function is stimulated by Asc Acid via an increase in collagen synthesis. © 1995 Wiley‐Liss, Inc.