Premium
In vitro structural and functional relationships between preosteoclastic and bone endothelial cells: A juxtacrine model for migration and adhesion of osteoclast precursors
Author(s) -
Formigli Lucia,
Orlandini Sandra Zecchi,
Benvenuti Susanna,
Masi Laura,
Pinto Antonio,
Gattel Valter,
Bernabei Pietro Antonio,
Robey Pamela Gehron,
CollinOsdoby Patricia,
Brandi Maria Luisa
Publication year - 1995
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041620206
Subject(s) - osteoclast , microbiology and biotechnology , bone resorption , progenitor cell , endothelial stem cell , biology , vitronectin , chemistry , immunology , in vitro , fibronectin , endocrinology , stem cell , extracellular matrix , biochemistry
The role of vascularization in the process of bone resorption has not been clarified. The interactions between vascular endothelium and osteoclast progenitors were analyzed using clonal cell lines of bone‐derived endothelial and preosteoclastic cells. Insulin‐like growth factor I is a major chemotactic stimulator of preosteoclastic cell migration mediated by bone endothelial cells. Osteoclast precursors rapidly adhered to bone endothelial monolayers. This phenomenon appeared to be cell‐specific and mediated through the binding of vitronectin and fibronection receptors to fibronectin. In addition, direct contact with bone endothelial cells induced osteoclast progenitors to differentiate into more mature elements, with the tendency to cluster together to form large multinucleated cells. These findings demonstrated specific in vitro interactions between bone endothelial cells and osteoclast progenitors, offering a new model for understanding the molecular mechanisms which direct the processes of osteoclast recruitment and ontogeny. © 1995 Wiley‐Liss, Inc.