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Tumor necrosis factor reduces proteoglycan synthesis in cultured endothelial cells
Author(s) -
Ramasamy S.,
Lipke D. W.,
McClain C. J.,
Hennig B.
Publication year - 1995
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041620114
Subject(s) - biglycan , proteoglycan , perlecan , tumor necrosis factor alpha , endothelial stem cell , decorin , glycosaminoglycan , chemistry , endothelium , extracellular matrix , cell , aggrecan , biochemistry , microbiology and biotechnology , biology , endocrinology , in vitro , pathology , medicine , articular cartilage , alternative medicine , osteoarthritis
Abstract Tumor necrosis factor (TNF)‐induced disruption of vascular endothelial barrier function may be due in part to alterations in proteoglycan metabolism. To test this hypothesis, confluent endothelial cell monolayers were exposed for 24 h to 500 or 1,000 U of TNF per mililiter of culture medium together with 20 μCi Na 2 35 SO 4 . HPLC anion‐exchange separation of proteoglycans secreted into media of control as well as TNF‐treated cultures revealed one major peak (representing 95% of total radioactivity) and one minor peak (representing 5% of total radioactivity), which eluted at 0.6 and 0.9 M NaCl, respectively. One single peak was obtained from control as well as TNF‐treated endothelial cell monolayers and eluted at 1.2 M NaCl. TNF treatment did not change the total quantity of radioactive proteoglycans secreted into the media but significantly decreased the amount of proteoglycans in endothelial cell monolayers. However, TNF treatment did not alter the size or glycosaminoglycan (GAG) composition of the proteoglycans either in the media or in the cell monolayers. In addition, mRNA levels of specific proteoglycans, perlecan and biglycan, were measured upon TNF treatment, using Northern analysis. TNF treatment caused a dose‐dependent decrease in mRNA levels for the biglycan in endothelial cultures. These results suggest that TNF decreases production of proteoglycans and alters normal endothelial cell proteoglycan metabolism which may be sufficient to impair endothelial barrier function. © 1995 Wiley‐Liss, Inc.