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Retinoids regulate expression of the retinol‐binding protein gene in hepatoma cells in culture
Author(s) -
Mourey Marie Sophie,
Quadro Loredana,
Panariello Luigi,
Colantuoni Vittorio
Publication year - 1994
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041600323
Subject(s) - retinoic acid , transfection , gene expression , gene , retinol binding protein , retinol , biology , endogeny , microbiology and biotechnology , regulation of gene expression , messenger rna , stimulation , retinoid , cell culture , biochemistry , endocrinology , genetics , vitamin
The expression of the gene coding for retinol‐binding protein has been studied in a system of cultured human hepatoma cells exposed to retinoids. We report that the gene is positively modulated by retinol and retinoic acid in a time‐ and dose‐dependent fashion. The stimulation at the mRNA level is paralleled by an increase of the corresponding protein that is secreted in the presence of the physiological ligand. An RBP‐CAT chimeric gene, introduced by transfection, is also responsive to the treatment, showing the gene dose‐dependency as the endogenous gene. These results demonstrate that retinoids up‐regulate the RBP gene and that the control takes place at transcriptional level. © 1994 Wiley‐Liss, Inc.