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cDNA from human ocular ciliary epithelium homologous to βig‐h3 is preferentially expressed as an extracellular protein in the corneal epithelium
Author(s) -
Escribano Julio,
Hernando Nati,
Ghosh Sikha,
CocaPrados Miguel,
Crabb John
Publication year - 1994
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041600314
Subject(s) - epithelium , corneal epithelium , homologous chromosome , microbiology and biotechnology , extracellular , complementary dna , ciliary body , biology , cilium , gene , genetics , neuroscience
Abstract The non‐pigmented ciliary epithelium is largely responsible for the formation of aqueous humor in the mammalian eye. To provide a basis for studies at the molecular level, a directional expression cDNA library was constructed in Uni‐ZAP XR vector from poly A + RNA of the human non‐pigmented ciliary epithelial derived ODM‐2 cell line. Fifty‐three cDNA clones were isolated from the library and characterized by partial sequence analysis. Approximately 49% of the clones exhibited homology with known genes in the GenBank/EMBL databases. The putative identification of these clones may reflect the transcriptional activity of the ODM‐2 cells in culture. One of the identified clones, ODM‐42‐I, was found to be specific and highly expressed in the corneal epithelium. This clone had an exact match with a recently discovered human gene, βig‐h3 (Skonier et al., 1992, DNA Cell Biol., 11 : 511–522), which codes a surface recognition protein, inducible by transforming growth factor beta (TGF‐β), and containing a putative binding site (RDG) for integrins. The ODM‐42‐I cDNA clone displays a distinctive pattern of expression found in the human eye, expressed almost exclusively in the cornea. Further studies, using sera from a synthetic peptide to the carboxy‐terminal region of ODM‐42‐I, reveal that the protein is heterogeneous in charge and is preferenctially expressed on the extracellular surface of corneal epithelial cells, and might share immunologic properties with integrins β1. © 1994 Wiley‐Liss, Inc.