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Effects of epoxyeicosatrienoic acids on 86 Rb uptake in renal epithelial cells
Author(s) -
Staudinger R.,
Abraham N. G.,
Schwartzman M. L.,
Escalante B.
Publication year - 1994
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041600109
Subject(s) - amiloride , chemistry , reabsorption , ouabain , epoxyeicosatrienoic acid , kidney , cytochrome p450 , arachidonic acid , renal sodium reabsorption , endogeny , renal physiology , furosemide , renal cortex , biochemistry , metabolism , enzyme , endocrinology , biology , sodium , renal function , organic chemistry
Epoxyeicosatrienoic acids (EETs) are arachidonic acid metabolites formed endogenously via the cytochrome P450 pathway in rat, rabbit, and human kidney. We characterized the effects of the four regioisomeric EETs on ion transport in the renal epithelial cell line, LLC‐PK 1 . Among the EETs, 14, 15‐EET was the most potent inhibitor of 86 Rb uptake. Its effect was concentration‐dependent (IC 50 = 75 nM) and stereoselective to the 14S, 15R‐EET. Experiments measuring 14, 15‐EET–induced 86 Rb uptake inhibition in the presence of inhibitors of Na + ‐K + ‐ATPase activity (ouabain), Na + ‐K + ‐Cl − cotransporter (furosemide), and Na + ‐H + exchanger (amiloride) suggested that 14, 15‐EET inhibits ion transport via an amiloride‐sensitive mechanism. These results, together with previous reports demonstrating their endogenous production in the kidney, suggest an important role for EETs, specifically 14, 15‐EET, in the regulation of ion and water reabsorption in the kidney and implicate their function in renal pathophysiology. © 1994 Wiley‐Liss, Inc.