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Human T lymphocytes synthesize the 92 kDa type IV collagenase (gelatinase B)
Author(s) -
Weeks Benjamin S.,
Schnaper H. William,
Handy Michael,
Holloway Eva,
Kleinman Hynda K.
Publication year - 1993
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041570326
Subject(s) - basement membrane , collagenase , type iv collagen , extravasation , secretion , microbiology and biotechnology , gelatinase , chemistry , gelatinase a , ht1080 , matrix metalloproteinase , biology , extracellular matrix , cell , biochemistry , immunology , laminin , enzyme
In order for T cells to exit the circulatory system, traverse the endothelial basement membrane, and arrive in target tissues, these cells must attach to and degrade basement membrane proteins. 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) has been shown to stimulate lymphoid cell adhesion to basement membrane components. We have used TPA to study the ability of human lymphoid cells to secrete type IV collagenases, enzymes capable of degrading basement membrane proteins. Here, we found that human primary T cells and H‐9 lymphoid cells synthesize the 92 kDa type IV collagenase (gelatinase B) and TPA stimulates the synthesis and secretion of this protease. Peak TPA‐stimulated gelatinase B secretion and mRNA accumulation were observed 9 hours after TPA treatment, while the peak adhesion to type IV collagen was observed only 3 hours after TPA treatment. The protein kinase C inhibitor, H‐7, inhibited TPA‐stimulated gelatinase B secretion. Both the primary T cells and H‐9 lymphoid cells also expressed the mRNA for the tissue inhibitor of metalloproteinase‐1 (TIMP‐1). These data demonstrate that TPA ‐ stimulated lymphoid cells adhere to type IV collagen and subsequently synthesize and secrete gelatinase B and TIMP‐1. We conclude that lymphoid cell extravasation may involve cellular employment of adhesion mechanisms prior to degradation of the matrix, which is similar to the process of extravasation used by metastatic cells. © 1993 Wiley‐Liss, Inc.