Premium
Inhibitors of vacuolar‐type H + ‐ATPase suppresses proliferation of cultured cells
Author(s) -
Manabe Tsukasa,
Yoshimori Tamotsu,
Henomatsu Nobuhiro,
Tashiro Yutaka
Publication year - 1993
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041570303
Subject(s) - bafilomycin , cell growth , cell culture , microbiology and biotechnology , atpase , in vitro , inhibitory postsynaptic potential , v atpase , biology , cell , growth inhibition , chemistry , biochemistry , enzyme , apoptosis , autophagy , endocrinology , genetics
We investigated effects of bafilomycin A 1 , a specific inhibitor of vacuolar‐type H + ‐ATPase (V‐ATPase), and its analogues on proliferation of various cultured cells. The proliferation of the various cell lines was suppressed by adding bafilomycin A 1 to the culture medium. This inhibitory effect appeared at a concentration of nanomolar order and was dose dependent. Although the suppression was reversible, the drug exerted not only suppression of the proliferation but also death to some cell lines. Drug concentration required for 50% inhibition of the cell proliferation during 48 h differed markedly depending on cell species and the sensitivity appears to increase by the transformation of the cells. Two derivatives of concanamycin A, an analogue of bafilomycin A 1 , also inhibited strongly V‐AT‐Pase in vitro and in vivo, and simultaneously cell proliferation. Two concanamycin A derivatives which have lost inhibitory effect on V‐ATPase lost inhibitory effect on cell proliferation as well. These results suggest that V‐ATPase is involved in the machinery maintaining the cell proliferation. © 1993 Wiley‐Liss, Inc.