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Fibroblast growth factor, epidermal growth factor, insulin‐like growth factors, and platelet‐derived growth factor‐BB stimulate proliferation of clonally derived porcine myogenic satellite cells
Author(s) -
Doumit Matthew E.,
Cook Douglas R.,
Merkel Robert A.
Publication year - 1993
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041570216
Subject(s) - platelet derived growth factor receptor , epidermal growth factor , growth factor , platelet derived growth factor , basic fibroblast growth factor , endocrinology , medicine , biology , cell growth , fibroblast growth factor , insulin like growth factor , cell culture , microbiology and biotechnology , receptor , biochemistry , genetics
Clonally derived cultures of porcine skeletal muscle satellite cells were developed. The mitogenic effects of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin‐like growth factors (IGF‐I and ‐II), and platelet‐derived growth factors (PDGF‐AA and ‐BB) were examined. Individually, bFGF, IGFs, and PDGF‐BB stimulated proliferation of porcine satellite cells grown in basal serum‐free medium or Minimum Essential Medium containing 2% fetal bovine serum (MEM‐2% FBS). EGF stimulated proliferation in MEM‐2% FBS, but neither EGF nor PDGF‐AA, were mitogenic when added to serum‐free medium. The interactions among bFGF, [GF, IGF‐I], and PDGF‐BB were examined in serum‐free medium, using growth factor concentrations shown in dose‐response experiments to induce maximal proliferative responses (10 ng/ml bFGF, EGF and PDGF‐BB, and 50 ng/ml IGF‐I). The combination of bFGF and IGF‐I dramatically increased proliferation, and IGF‐I also synergized with EGF to increase proliferation. EGF, IGF‐I and bFGF interacted with PDGF‐BB to stimulate proliferation. With the exception of EGF and bFGF, combinations of two growth factors typically resulted in greater than additive responses. Simultaneous exposure of satellite cells to bFGF, PDGF‐BB, EGF, and IGF‐I produced a fivefold increase in DNA compared to cells grown in basal serum‐free medium. Elimination of EGF did not reduce the mitogenic response, yet removal of IGF‐I, bFGF, or PDGF‐BB reduced proliferation by ∼40, 20, and 10%, respectively. These mitogens are likely physiological regulators of porcine satellite cell activity. © 1993 Wiley‐Liss, Inc.