Premium
Cell cycle activity and expression of prohibitin mRNA
Author(s) -
Roskams A. Jane I.,
Friedman Varda,
Wood Carla M.,
Walker Lisa,
Owens Garrison A.,
Stewart David A.,
Altus Michael S.,
Danner David B.,
Liu XiaoTie,
McClung J. Keith
Publication year - 1993
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041570211
Subject(s) - prohibitin , cell cycle , messenger rna , microbiology and biotechnology , biology , cell , chemistry , mitochondrion , biochemistry , gene
Prohibitin, a novel intracellular antiproliferative protein, blocks entry into the S phase of the cell division cycle when its mRNA is microinjected into normal fibroblasts or HeLa cells. To learn more about the interaction between prohibitin and the cell cycle, we studied the effect of microinjecting prohibitin mRNA at different points during the transition from G 0 to S phase and analyzed prohibitin mRNA and protein levels in different parts of the cell cycle. The antiproliferative activity of microinjected prohibitin mRNA is high in G 0 /G 1 and falls as cells approach S phase. Prohibitin mRNA and protein levels are high in G 1 , fall with S phase, rise again in G 2 , and fall in M. Together, these findings suggest that endogenous prohibitin contributes to the control of the G 1 to S transition in cycling cells in a complex manner, which involves both a transcriptional and posttranslational mechanism. © 1993 Wiley‐Liss, Inc.